董国锋¹，赵小亮¹，郑晓杰^2*，赵海军¹，胡昙昙²，李彦坡²，巩卫琪³，邓文彬³

（1.兰州理工大学生命科学与工程学院，兰州730050；2.温州市农业科学研究院/浙南作物育种重点实验室，温州325006；3.浙江铁枫堂健康科技有限公司，温州325604）

摘要：该研究以雁荡山铁皮石斛叶为原料，以多酚提取量为指标，采用超声协同酶法提取铁皮石斛叶多酚，通过单因素和响应面实验优化提取工艺。结果表明，最佳提取工艺为：乙醇体积分数65%、超声功率360 W、液料比50∶1mL/g、超声时间40min、超声温度58℃、纤维素酶添加量4mg/g、提取2次，在此条件下测得铁皮石斛叶多酚的提取量为38.23mg/g。该研究可为铁皮石斛叶的进一步开发利用提供理论依据。

关键词：铁皮石斛叶；超声波；多酚；纤维素酶

中图分类号：R284.2 文献标识码：A 文章编号：1674-506X（2025）02-0034-0009

Process Optimization of Polyphenol Extraction from Yandang Mountain Dendrobium officinale Leaves by Ultrasonic-assisted Enzymatic Method

DONG Guofeng¹, ZHAO Xiaoliang¹, ZHENG Xiaojie^2*, ZHAO Haijun¹, HU Tantan², LI Yanpo², GONG Weiqi³, DENG Wenbin³

(1.College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China;

2.Wenzhou Academy of Agricultural Sciences/Key Laboratory of Crop Breeding in Southern Zhejiang Province, Wenzhou 325006, China;

3.Zhejiang Tiefentang Health Technology Co., Ltd., Wenzhou 325604, China)

Abstract: In this research, Dendrobium officinale leaves from Yandang mountain were used as raw materials, using the polyphenol extraction amount as the indicator and Dendrobium officinale leaves polyphenol was extracted by ultrasonic assisted enzymatic method, extraction process was optimized through single factor and response surface tests. The results showed that the best extraction conditions were: ethanol volume fraction 65%, ultrasonic power 360 W, liquid-solid ratio 50:1 mL/g, ultrasonic time 40 min, ultrasonic temperature 58 ℃, cellulase adding dosage 4 mg/g, extraction twice. Under these conditions, the measured extraction amount of polyphenols from Dendrobium officinale leaves was 38.23 mg/g. This research can provide theoretical basis for the further development and utilization of Dendrobium officinale leaves.

Keywords: Dendrobium officinale leaves; ultrasound; polyphenols; cellulase

doi: 10.3969/j.issn.1674-506X.2025.02-005